PODS® Human CXCL10

Code Description Price Qty
PPH127-50 PODS® Human CXCL10, 50 million $160.00
PPH127-250 PODS® Human CXCL10, 250 million $495.00
PPH127-1000 PODS® Human CXCL10, 1 billion $1,650.00
PODS® co-crystals
PODS® co-crystals

PODS® Technology

PODS® proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS® co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS® Technology page.

Product Description

The product contains the polyhedrin protein co-crystalized with Human CXCL10. Also known as Interferon γ-induced protein 10 (IP-10), CXCL10 is a chemokine secreted by monocytes, endothelial cells and fibroblasts in response to interferon γ (IFN-γ). CXCL10 functions as a chemoattractant for activated T cells, monocytes, dendritic, and Natural Killer (NK) cells that express the G protein-coupled receptor CXCR3. It is an important factor in autoimmune diseases such as Hashimoto's thyroiditis, Graves' disease, and Type 1 diabetes mellitus.

Usage Recommendation

PODS® co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® co-crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS® co-crystals in place of 3.3 µg of standard growth factor as a starting point.

To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.

Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

AA Sequence

MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGFMNQTA ILICCLIFLT LSGIQGVPLS RTVRCTCISI SNQPVNPRSL EKLEIIPASQ FCPRVEIIAT MKKKGEKRCL NPESKAIKNL LKAVSKERSK RSP

Alternative Names

Interferon gamma-inducible protein 10, IP-10, Interferon gamma induced factor, gIP-10, IFI10, INP10

Research Use Only

This product is for Research Use Only.

Product Details
Length

143 aa

Molecular Weight

32.1 kDa

Structure

Dimer

Source

Spodoptera frugiperda (Sf9) cell culture

Accession Number

P02778

Endotoxin Level

<0.06 EU/ml as measured by by gel clot LAL assay

Formulation

PODS® were lyophilized from a volatile solution

Reconstitution

PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting.

PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).

Stability and Storage

Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended.

References

Fasséli Coulibaly, Elaine Chiu, Keiko Ikeda, Sascha Gutmann, Peter W. Haebel, Clemens Schulze-Briese, Hajime Mori, and Peter Metcalf. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.

Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.

Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.

Satoshi Abe, Hiroshi Ijiri, Hashiru Negishi, Hiroyuki Yamanaka, Katsuhito Sasaki, Kunio Hirata, Hajime Mori, and Takafumi Ueno. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.