PODS® mCherry

Code Description Price Qty
PP304-50 PODS® mCherry, 50 million $160.00
PP304-250 PODS® mCherry, 250 million $495.00
PP304-1000 PODS® mCherry, 1 billion $1,650.00
PODS® mCherry co-crystals
PODS® mCherry co-crystals

PODS® Technology

PODS® proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS® co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS® Technology page.

Product Description

The product contains polyhedrin protein co-crystalized with mCherry. mCherry is a uniquely versatile biomarker and, encased into PODS® crystals, offers a simple way to visualize und localize PODS® crystals embedded in biomaterials, such as hydrogels and scaffolds, using fluorescence microscopy. PODS® mCherry crystals can be excited at 587 nm and optimally detected at 610 nm, compatible with commonly available filter sets.

Usage Recommendation

PODS® mCherry crystals display the same physical properties as other PODS® growth factor products. While PODS® mCherry behave in the same way as other PODS® co-crystals, they differ in that they do not contain a cargo protein that elicits effects on cells. Instead of this, they have fluorescent proteins (mCherry) embedded. PODS® mCherry can be used analogous to PODS® Empty as an inert control, but the primary purpose is to enable visualization and localization of PODS® crystals in cell culture, e.g. in 3D scaffolds, hydrogels and other biomaterials, utilizing fluorescence microscopy.

Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

AA Sequence

MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGFMVSKG EEDNMAIIKE FMRFKVHMEG SVNGHEFEIE GEGEGRPYEG TQTAKLKVTK GGPLPFAWDI LSPQFMYGSK AYVKHPADIP DYLKLSFPEG FKWERVMNFE DGGVVTVTQD SSLQDGEFIY KVKLRGTNFP SDGPVMQKKT MGWEASSERM YPEDGALKGE IKQRLKLKDG GHYDAEVKTT YKAKKPVQLP GAYNVNIKLD ITSHNEDYTI VEQYERAEGR HSTGGMDELY K

Alternative Names

M Cherry, mCherry protein, mCherry fluorescent protein

Research Use Only

This product is for Research Use Only.

Product Details
Length

237 aa

Molecular Weight

31.9 kDa

Structure

Monomer

Source

Spodoptera frugiperda (Sf9) cell culture

Formulation

PODS® were lyophilized from a volatile solution

Reconstitution

PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting.

PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).

Stability and Storage

Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended.

References

Fasséli Coulibaly, Elaine Chiu, Keiko Ikeda, Sascha Gutmann, Peter W. Haebel, Clemens Schulze-Briese, Hajime Mori, and Peter Metcalf. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.

Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.

Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.

Satoshi Abe, Hiroshi Ijiri, Hashiru Negishi, Hiroyuki Yamanaka, Katsuhito Sasaki, Kunio Hirata, Hajime Mori, and Takafumi Ueno. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.