PODS® Mouse GM-CSF

Code Description Price Qty
PPM15-50 PODS® Mouse GM-CSF, 50 million $170.00
PPM15-250 PODS® Mouse GM-CSF, 250 million $490.00
PPM15-1000 PODS® Mouse GM-CSF, 1 billion $1,630.00
PODS® co-crystals
PODS® co-crystals

PODS® Technology

PODS® proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS® co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS® Technology page.

Product Description

The product contains polyhedrin protein co-crystalized with mature mouse Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). GM-CSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells including granulocytes, eosinophils, erythrocytes, and macrophages. Mature mouse GM-CSF shares 54% and 69% amino acid sequence identity with human rat GM-CSF respectively. The activity of the human and mouse GM-CSF is species-specific. Rat GM-CSF is fully active on mouse cells, but mouse GM-CSF is weakly active on rat cells. GM-CSF is involved in immune response, allergy, inflammatory processes, angiogenesis and, autoimmunity.

Usage Recommendation

PODS® co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® co-crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS® co-crystals in place of 3.3 µg of standard growth factor as a starting point.

To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.

Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

AA Sequence

Immobilization tag shown in blue.

MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGFAPTRS PITVTRPWKH VEAIKEALNL LDDMPVTLNE EVEVVSNEFS FKKLTCVQTR LKIFEQGLRG NFTKLKGALN MTASYYQTYC PPTPETDCET QVTTYADFID SLKTFLTDIP FECKKPGQK

Alternative Names

Granulocyte-Macrophage Colony-Stimulating Factor, GMCSF, Colony Stimulating Factor 2 (CSF-2), Molgramostin, Sargramostim

Research Use Only

This product is for research use only.

Product Details
Length

169 aa

Molecular Weight

19.3 kDa

Structure

Monomer

Source

Spodoptera frugiperda (Sf9) cell culture

Accession Number

P01587

Formulation

PODS® were lyophilized from a volatile solution

Reconstitution

PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting.

PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).

Stability and Storage

Upon receipt, store at 4®C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended.

References

Fasséli Coulibaly, Elaine Chiu, Keiko Ikeda, Sascha Gutmann, Peter W. Haebel, Clemens Schulze-Briese, Hajime Mori, and Peter Metcalf. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.

Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.

Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.

Satoshi Abe, Hiroshi Ijiri, Hashiru Negishi, Hiroyuki Yamanaka, Katsuhito Sasaki, Kunio Hirata, Hajime Mori, and Takafumi Ueno. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.