Recombinant Human TGF β3 (plant produced)
Description
This product has been manufactured in plants. It has levels of bioactivity that compare very favourably with competitor products.
Transforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in most cell types. The TGF-β family of proteins signal through serine/threonine kinase receptors. TGF-β isoforms (TGF-β1, -β 2, and -β 3) have overlapping, yet distinct biological actions in developing and adult tissues. TGF-β3 is an important factor in regulating cell adhesion and accelerating wound repair. TGF-β3 also functions during osteoblast proliferation, chemotaxis, and collagen synthesis.
Activity Assay
TGF-β3 responsive SBE Reporter HEK293 cells were incubated with increasing concentrations of TGF-β3 for 18 hours. Luciferase activity was measured using the ONE-Step Luciferase Assay System (BPS Bioscience #60690). The results are shown as fold induction of SBE luciferase reporter expression in relation to the activity of cells. EC50 for bioactivity of TFG-β3 recorded at 0.109
AA Sequence
MALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS
Alternative Names
Transforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in most cell types. The TGF-β family of proteins signal through serine/threonine kinase receptors. TGF-β isoforms (TGF-β1, -β 2, and -β 3) have overlapping, yet distinct biological actions in developing and adult tissues. TGF-β3 is an important factor in regulating cell adhesion and accelerating wound repair. TGF-β3 also functions during osteoblast proliferation, chemotaxis, and collagen synthesis.
| Product Details | |
|---|---|
| Length |
113 / 226 aa |
| Molecular Weight |
12.9 / 25.7 kDa |
| Structure |
Dimer |
| Source |
Nicotiana tabacum |
| Accession Number |
P10600 |
| Purity |
≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level |
≤1.00 EU/µg as measured by kinetic LAL |
| Formulation |
Lyophilised in 20% EtOH, 0.12% Acetic acid pH4.0. |
| Reconstitution |
10mM HCl |
| Stability and Storage |
At least 12 months from the date of receipt when stored at -20°C as supplied. |
Competitor comparison
Comparison of CellGS plant-produced TGF-β3 against two competitor products. TGF-β3 responsive SBE Reporter HEK293 cells were incubated with increasing concentrations of TGF-β3 for 18 hours. Luciferase activity was measured using the ONE-Step Luciferase Assay System (BPS Bioscience #60690). The results are shown as fold induction of SBE luciferase reporter expression in relation to the activity of cells. EC50 for bioactivity of TFG-β3 recorded at 0.109
