Exo-spin™ maxi

Product description

The Exo-spin™ maxi is the largest-volume SEC column in the Exo-spin range, designed for exosome and extracellular vesicle (EV) purification from cell culture media, saliva, urine and blood. With a XX ml bed volume and 30 nm pore size, the midi column achieves 99.5% protein removal. Blood samples (plasma, sera) up to 5 ml can be loaded directly; larger volumes of dilute samples such as culture media (up to 500 ml) are first concentrated using Exo-spin buffer (EX06) or EVlution TFF systems. The simple 5-step protocol completes in under two hours with no ultracentrifuge required. Each column is reusable up to five times

Exo-spin™ products provide a proven, rapid and reliable way of generating high-quality purified exosomes suitable for a range of research applications.

Exo-spin™ technology is based on size exclusion chromatography (SEC), which is reliable for exosome isolation. A concentration step (using Exo-spin buffer or concentration columns) is recommended if a starting sample has low exosome-content, such as cell culture media, urine, saliva etc. Without this important step, a lower exosome recovery will be obtained. Exo-spin™ provides a simple protocol which allows you to purify your sample in less than 2 hours with consistent and reliable results.

The EX08 Exo-spin™ product contains packed and equilibrated ready-to-use SEC columns.  5 ml of blood samples (plasma and sera) may be added onto the column directly.  

Exo-spin™ column and resin pore size

The column bed volume in EX08 Exo-spin™ midi-columns is XX ml, allowing for 5 ml of volume to be loaded onto each column.

The pore size within the resin is approximately 30 nm to attain a highly pure exosome elution. All other molecules (e.g. proteins, lipids) which are smaller than 30 nm will enter into the pores and remain trapped in the column. 

Highest recovery and purity

Exosomes in a range 30-200 nm will elute in the first fraction. 99.5% other proteins and lipids will be retained in the column and will elute later than the exosomes, ensuring a highly pure sample ready for downstream application.

Reproducibility

All our columns are manufactured in our laboratory to ensure a high reproducibility between each lot. As proof of reproducibility and batch-to-batch consistency, a large number of peer-reviewed scientific papers have been published describing the use of Exo-spin™.

Storage

Upon receipt, store purification columns and Exo-spin™ Buffer at 4°C. All other components should be stored at room temperature (15°C - 25°C).

Frequently Asked Questions (FAQs)

For any additional questions, please refer to FAQs document below.

If you are just starting to work with exosomes, consider our starter pack and get  in touch with us for advice through the chat function on the bottom right

We designed the ideal starter pack to guide your exosome research. The starter pack includes the exosome purification kit of your choice, exosome validated antibodies, and NTA profiling analysis. Complete details can be found in the product page here.

See other Exo-spin™ columns