PODS® Human Ephrin-A3
PODS® Technology
PODS® proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS® co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS® Technology page.
Product Description
The product contains the polyhedrin protein co-crystalized with Human Ephrin-A3. Ephrin-A3 is a member of Ephrin-A famil, and it is also known as EHK1-L, EFL-2, and LERK-3. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A3 expression can be up- or down-regulated by hypoxia in the hippocampus or vascular endothelial cells, respectively. Ephrin-A3 interaction with EphA receptors induces neurite growth cone collapse and the repulsion of migrating axons, which is important for the accurate migration of axons during CNS development.
Usage Recommendation
PODS® are pure protein co-crystals consisting of polyhedrin, a structural scaffold protein, and a cargo protein. Under the action of proteases, which degrade the scaffold protein, PODS provide sustained release of the cargo protein. Any cargo growth factor molecule contained within PODS is not available to cells and not bioactive. Once released, growth factors become available to bind cells and are bioactive. The concentration to which a growth factor accumulates in cell culture media (or in-vivo environment) will depend on the amount of cargo (contained in PODS) added, the rate of cargo release, and the subsequent rate of degradation of the released cargo protein. As a rule of thumb, in the presence of 10% serum, peak levels of available growth factors released from PODS are reached within 24-48 hours. Typically, at this point 20% of the growth factor cargo initially contained within the PODS is present in a soluble form and available to bind cells. For example, if PODS containing 100 ng of cargo are added to 10 ml of cell culture media containing 10% serum, it can be expected that 20 ng will be released after 24 hours to give a concentration of available growth factor of 2 ng/ml. The concentration that you need for a particular application will likely be lower than the equivalent conventional growth factor. This is because PODS are better at maintaining minimum growth factor concentrations. Pre-incubating PODS with serum for 24 hours prior to culture will ensure that available growth factor is immediately present. Ultimately, the amount of PODS growth factor that is optimal for a particular experiment should be optimized empirically.
Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
AA Sequence
Immobilization tag shown in blue.
MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGFNRHAV YWNSSNQHLR REGYTVQVNV NDYLDIYCPH YNSSGVGPGA GPGPGGGAEQ YVLYMVSRNG YRTCNASQGF KRWECNRPHA PHSPIKFSEK FQRYSAFSLG YEFHAGHEYY YISTPTHNLH WKCLRMKVFV CCASTSHSGE KPVPTLPQFT MGPNVKINVL EDFEGENPQV PKLEKSISGT SPKREHLPLA VGIAFFLMTF LAS
Alternative Names
EphrinA3, EFL2, EFL-2, EFNA3, Ehk1-L, EPH-related receptor tyrosine kinase ligand 3, EPLG3EHK1 ligand, LERK-3, LERK3, ligand of eph-related kinase 3
Research Use Only
This product is for Research Use Only.
PODS universal cargo antibody and ELISA
Use the PODS universal cargo antibody (PUCA) for western blots and other assays to monitor the release of cargo proteins released from PODS. This works with all PODS proteins with few exceptions. In addition, an optimized PUCA ELISA is available
| Product Details | |
|---|---|
| Length |
253 aa |
| Molecular Weight |
28.7 kDa |
| Structure |
Monomer |
| Source |
Spodoptera frugiperda (Sf9) cell culture |
| Accession Number |
AAA52368 |
| Formulation |
PODS® were lyophilized from a volatile solution |
| Reconstitution |
Ensure the PODS® are resuspended in buffer by pipetting up and down immediately before aliquoting. PODS® may be reconstituted at 100 ug/ml in water. 20% glucose has a buoyant density closer to PODS® and can be useful for slowing sedimentation when aliquoting. PODS® are highly stable when stored in aqueous solution (pH range 6 - 8). |
| Stability and Storage |
Upon receipt, store at 4®C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended. |
References
Fasséli Coulibaly, Elaine Chiu, Keiko Ikeda, Sascha Gutmann, Peter W. Haebel, Clemens Schulze-Briese, Hajime Mori, and Peter Metcalf. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.
Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.
Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.
Satoshi Abe, Hiroshi Ijiri, Hashiru Negishi, Hiroyuki Yamanaka, Katsuhito Sasaki, Kunio Hirata, Hajime Mori, and Takafumi Ueno. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.
