Recombinant Human Artemin

Code Description Price Qty
GFH23-5 Recombinant Human Artemin, 5 μg £50.00
GFH23-20 Recombinant Human Artemin, 20 μg £120.00
GFH23-100 Recombinant Human Artemin, 100 μg £310.00
GFH23-100x10 Recombinant Human Artemin, 100 μg x 10 £1,375.00
GFH23-1000 Recombinant Human Artemin, 1 mg £1,375.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Artemin has a predicted Mw of 24.2 kDa (each monomer is 12.1 kDa).

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PODS Growth Factors

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Description

Artemin is a Neurotrophin member of the Glial cell line-derived Neurotrophic Factor (GDNF) ligand family. Artemin is highly expressed in the adult pituitary gland, placenta, and trachea, and shows low level expression in the brain, spinal cord, and peripheral tissues. Artemin signals through the RET receptor and GDNF family receptor α 3 (GFR α 3) co-receptor complex to support neuronal survival.

Activity Assay

Human Artemin is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation assay the SH-SY5Y cell line, was found to be 4 - 8 ng/ml. The activity can also be determined by its ability to promote survival and neurite outgrowth.

AA Sequence

AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG

Alternative Names

ARTN, ENOVIN, NBN1, Enovin3, neublastin, EVN1, neurotrophic factor, neurotrophic factor artemin
Product Details
Length 113 / 226 aa
Molecular Weight 12.1 / 24.2 kDa
Structure Dimer
Source E. coli
Accession Number Q5T4W7
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium citrate, 25 mM sodium chloride, pH 4.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.