Recombinant Human BMP-2

Code Description Price Qty
GFH53-2 Recombinant Human BMP-2, 2 μg £50.00
GFH53-10 Recombinant Human BMP-2, 10 μg £120.00
GFH53-100 Recombinant Human BMP-2, 100 μg £375.00
GFH53-100x10 Recombinant Human BMP-2, 100 μg x 10 £1,950.00
GFH53-1000 Recombinant Human BMP-2, 1 mg £1,950.00
Induced alkaline phosphatase in ATDC5 cells assay for Human BMP-2. Alkaline phosphatase was measured to calculate the ED50, which is as expected less than 250 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human BMP-2 has a predicted Mw of 26.0 kDa (each monomer is 13.0 kDa).

Achieve Purchasing Synergy

Request a quote at quotes@cellgs.com for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order (offer excludes the largest pack size - please contact us).

PODS Growth Factors

The product is current not available as PODS™, please check back in the future.

Description

Bone Morphogenic Protein 2 (BMP-2) is a member of the Bone Morphogenetic Protein (BMP) family. These proteins are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. BMP-2 functions as a potent inducer of bone and cartilage development. Active BMP-2 consists of forming a homodimer or a heterodimer with a related BMP, such as BMP-7. BMP-2 signals through type I and type II receptor tyrosine kinases in conjuction with SMAD proteins to directly promote osteoblast differentiation. BMP-2 is also important during cardiac development and supports epicardial cell migration.

Activity Assay

Human BMP-2 is fully biologically active when compared to standard. The activity is determined by the alkaline phosphatase activity induced in ATDC5 cells and it is typically less than 250 ng/ml. This corresponds to an expected specific activity of 4.0 x 103 units/mg.

AA Sequence

MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Alternative Names

Bone morphogenetic protein 2, bone morphogenetic protein 2A, BMP-2A, BMP2, BMP2A
Product Details
Length 115 / 230 aa
Molecular Weight 13.0 / 26.0 kDa
Structure Dimer
Source E. coli
Accession Number P12643
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.

Citations

Bao W, Gao M, Cheng Y, Lee HJ, Zhang Q, Hemingway S, Luo Z, Krol A, Yang G, An J. Biomodification of PCL Scaffolds with Matrigel, HA, and SR1 Enhances De Novo Ectopic Bone Marrow Formation Induced by rhBMP-2. (2015).

Documents

GFH53 Datasheet.pdfMSDS Recombinant Proteins.pdf