Recombinant Human BMP-4
PODS Growth FactorsThis growth factor is also available as PODS™ for sustained release, see here.
DescriptionBone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein (BMP) family and functions in a variety of developmental processes. BMP-4 is a critical element involved in the culture and differentiation of stem cells, including differentiation in mesoderm (cardiac and skeletal muscle cells and early hematopoietic cells) and ectoderm (neural cell types and skin cell types) from pluripotent stem cells (iPSC and ESC).
Activity AssayHuman BMP-4 is fully biologically active when compared to standard. The activity is determined by the alkaline phosphatase activity induced in ATDC5 cells and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.
AA SequenceSPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
Alternative NamesBone morphogenetic protein 4, bone morphogenetic protein 2B, BMP-2B, BMP4, BMP2B
|Length||116 / 232 aa|
|Molecular Weight||13.1 / 26.2 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.