Recombinant Human BMP-4

Code Description Price Qty
GFH212-5 Recombinant Human BMP-4, 5 μg £120.00
GFH212-20 Recombinant Human BMP-4, 20 μg £375.00
GFH212-100 Recombinant Human BMP-4, 100 μg £1,295.00
GFH212-100x10 Recombinant Human BMP-4, 100 μg x10 £3,850.00
GFH212-1000 Recombinant Human BMP-4, 1 mg £3,850.00
Induced alkaline phosphatase in ATDC5 cells assay for Human BMP-4. Alkaline phosphatase was measured to calculate the ED50, which is as expected less than 15 ng/ml.

PODS Growth Factors

This growth factor is also available as PODS™ for sustained release, see here.

Description

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein (BMP) family and functions in a variety of developmental processes. BMP-4 is a critical element involved in the culture and differentiation of stem cells, including differentiation in mesoderm (cardiac and skeletal muscle cells and early hematopoietic cells) and ectoderm (neural cell types and skin cell types) from pluripotent stem cells (iPSC and ESC).

Activity Assay

Human BMP-4 is fully biologically active when compared to standard. The activity is determined by the alkaline phosphatase activity induced in ATDC5 cells and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.

AA Sequence

SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR

Alternative Names

Bone morphogenetic protein 4, bone morphogenetic protein 2B, BMP-2B, BMP4, BMP2B
Product Details
Length 116 / 232 aa
Molecular Weight 13.1 / 26.2 kDa
Structure Dimer
Source CHO
Accession Number P12644
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.