Recombinant Human CTGF
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human CTGF has a predicted Mw of 11.2 kDa.
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DescriptionConnective Tissue Growth Factor (CTGF) is a mitogen that is secreted by vascular endothelial cells in response to basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). CTGF promotes cell growth, migration, adhesion, and survival of endothelial cells. CTGF is also important during osteogenesis, chondrogenesis, and skeletogenesis. CTGF has an insulin-like growth factor binding protein (IGFBP) domain, a thrombospondin type 1 repeat (TSR) domain, and a C-terminal cysteine knot motif.
Activity AssayHuman CTGF is fully biologically active when compared to standard. The activity is determined by the dose-dependent induction of HUVEC cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity of 500 units/mg.
AA SequenceMGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
Alternative NamesConnective Tissue Growth Factor, IGF-binding protein 8, IGFBP8, IGFBP-8, IBP-8, CCN2, HCS24,insulin-like growth factor-binding protein HCS24, hypertrophic chondrocyte-specific protein 24, NOV2, CCN family member, 2MGC102839
|Molecular Weight||11.2 kDa|
|Purity||≥90% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.