Recombinant Human EG-VEGF (Animal-Free)

Code Description Price Qty
GFH7AF-5 Recombinant Human EG-VEGF (AF), 5 μg £55.00
GFH7AF-20 Recombinant Human EG-VEGF (AF), 20 μg £127.50
GFH7AF-100 Recombinant Human EG-VEGF (AF), 100 μg £320.00
GFH7AF-100x10 Recombinant Human EG-VEGF (AF), 100 μg x 10 £1,525.00
GFH7AF-1000 Recombinant Human EG-VEGF (AF), 1 mg £1,525.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human EG-VEGF has a predicted Mw of 9.7 kDa.

Achieve Purchasing Synergy

Request a quote at quotes@cellgs.com for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order (offer excludes the largest pack size - please contact us).

PODS Growth Factors

The product is current not available as PODS™, please check back in the future.

Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Endocrine Gland-derived Vascular Endothelial Growth Factor (EG-VEGF) is an angiogenic growth factor that is expressed in the ovaries, testis, adrenal, and placental tissues. EG-VEGF has mitogenic, chemoattractive, and antiapoptotic functional roles. EG-VEGF signaling is mediated through binding the G protein-coupled receptors prokineticin receptor 1 (PKR1) and prokineticin receptor 2 (PKR2). Polycystic ovaries display strong EG-VEGF expression that is associated with increased angiogenesis and cyst formation, which could lead to the formation of polycystic ovary syndrome and infertility.

Activity Assay

Human EG-VEGF is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of MIA PaCa-2 cells and it is typically 1 - 4 μg/ml. This corresponds to an expected specific activity of 1 x 103 units/mg. There is no data currently available.

AA Sequence

AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF

Alternative Names

Endocrine Gland-derived Vascular Endothelial Growth Factor, prokineticin 1, PROK1
Product Details
Length 86 aa
Molecular Weight 9.7 kDa
Structure Monomer
Source E. coli
Accession Number P58294
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.