Recombinant Human EGF (Animal-Free)

Code Description Price Qty
GFH26AF-100 Recombinant Human EGF (AF), 100 μg £55.00
GFH26AF-500 Recombinant Human EGF (AF), 500 μg £127.50
GFH26AF-1000 Recombinant Human EGF (AF), 1 mg £192.50
GFH26AF-500x10 Recombinant Human EGF (AF), 500 μg x 10 £565.00
GFH26AF-5000 Recombinant Human EGF (AF), 5 mg £565.00
Induced proliferation of BALB/c 3T3 cells assay for Human EGF. Cell proliferation was measured to calculate the ED50, which is as expected between 20 - 100 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human EGF has a predicted Mw of 6.2 kDa.

Achieve Purchasing Synergy

Request a quote at quotes@cellgs.com for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order (offer excludes the largest pack size - please contact us).

PODS Growth Factors

This growth factor is also available as PODS™ for sustained release, see here.

Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR). EGF is overexpressed in multiple tumor cell lines and promotes resistance to chemotherapy and radiation treatments.

Activity Assay

Human EGF (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of 3T3 cells proliferation and it is typically between 20 - 100 pg/ml. This corresponds to an expected specific activity of 1 x 107 units/mg.

AA Sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Alternative Names

Epidermal Growth Factor, pro-epidermal growth factor, urogastrone, URG, β-urogastrone, HOMG4
Product Details
Length 53 aa
Molecular Weight 6.2 kDa
Structure Monomer
Source E. coli
Accession Number P01133
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.