Recombinant Human ENA-78 / CXCL5
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DescriptionEpithelial-derived neutrophil-activating peptide 78 (ENA-78), also known as CXCL5, is a chemokine that recruits neutrophils, promotes angiogenesis, and stimulates connective tissue remodelling. ENA-78 production is stimulated by Interleukin-1 (IL-1) or tumor Necrosis Factor α (TNF-α), and signals through the chemokine receptor CXCR2. ENA-78 with 5 - 78 aminoacids is one of three naturally occuring ENA-78 variants in which the N-terminus has been truncated.
Activity AssayHuman ENA-78 is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of primary human neutrophils starting at a concentration of 10 ng/ml. This corresponds to an expected specific activity of 1 x 105 units/mg. There is no data currently available.
AA SequenceAAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN
Alternative NamesCXCL5, epithelial-derived neutrophil-activating protein 78, ENA78, ENA 78
|Molecular Weight||8.1 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.