Recombinant Human Exodus-2 / CCL21
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Exodus-2 has a predicted Mw of 12.3 kDa, but runs approximately 16 kDa on SDS-PAGE.
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DescriptionExodus-2, also known as CCL21, is a chemokine that is strongly produced in the human lymph nodes and spleen. Exodus-2 signals through the chemokine receptor CCR7 to regulate thymocyte and activated T cell migration, but is not chemotactic for monocytes. Exodus-2 also mediates the homing of lymphocytes to the lymphatic system. Human and mouse Exodus-2 share > 85% amino acid identity.
Activity AssayActivity to be determined.
AA SequenceSDGGAQDCCL KYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGC RKDRGASKTG KKGKGSKGCK RTERSQTPKG P
Alternative NamesCCL21, 6Ckine, secondary lymphoid tissue chemokine, SLC
|Molecular Weight||12.3 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.