Recombinant Human FGF-10 (Animal-Free)
Induced proliferation of 4MBr-5 cells assay for Human FGF-10. Cell proliferation was measured to calculate the ED50, which is as expected less than 200 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human FGF-10 has a predicted Mw of 19.3 kDa.
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PODS® Growth FactorsThis growth factor is also available as PODS® for sustained release, see here.
Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionFibroblast Growth Factor 10 (FGF-10) is a growth factor that is important during embryonic development, especially during lung, limb, brain, heart, and kidney morphogenesis. FGF-10 is expressed in mesenchymal cells and facilitates epithelial-mesenchymal signaling through binding the epithelially expressed FGF receptor 2b (FGFR2b). FGF-10 also functions as a mitogen for keratinizing epidermal cells and induces the migration and invasion of cancer cells.
Activity AssayHuman FGF-10 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by a proliferation assay using 4MBr-5 cells and it is typically less than 200 ng/ml. This corresponds to an expected specific activity of 5.0 x 103 units/mg.
AA SequenceMLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS
Alternative NamesFibroblast Growth Factor 10, FGF10, FGF 10, Keratinocyte Growth Factor 2
|Molecular Weight||19.3 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.