Recombinant Human FGF-2 (147)

Code Description Price Qty
GFH28-10 Human FGF-2 (147), 10 μg $65.00
GFH28-50 Human FGF-2 (147), 50 μg $165.00
GFH28-100 Human FGF-2 (147), 100 μg $295.00
GFH28-1000 Human FGF-2 (147), 1 mg $1,310.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human FGF-2 (147) has a predicted Mw of 16.5 kDa.

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PODS® Growth Factors

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Description

Fibroblast Growth Factor 2 (FGF-2) is expressed by endothelial cells and is a mediator of angiogenesis. FGF-2 also has cardioprotective functions during heart injury. The application of FGF-2 is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degredation of the full length FGF-2 N-terminus results in a truncated FGF-2 147 amino acids protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of FGF-2 within the cell, but do not affect the biological activity of FGF-2. Therefore, there are no detectable differences in biological activity between the full length FGF-2 154 amino acids and the truncated FGF-2 147 amino acids recombinant proteins.

Activity Assay

Human FGF-2 (147) is fully biologically active when compared to standard. The activity is determined by the dose-dependent induced proliferation of NR6R 3T3 cells and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.

AA Sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Alternative Names

Fibroblast Growth Factor 2, FGF2, FGF 2, HBGF-2, basic fibroblast growth factor, heparin-binding growth factor 2, FGFB, BFGF, bFGF, prostatropin
Product Details
Length 147 aa
Molecular Weight 16.5 kDa
Structure Monomer
Source E. coli
Accession Number P09038
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.