Recombinant Human FGF-8 (Animal-Free)
Induced proliferation of BALB/c 3T3 cells assay for Human FGF-8. Cell proliferation was measured to calculate the ED50, which is as expected less than 150 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human FGF-8 has a predicted Mw of 22.5 kDa.
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Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionFibroblast Growth Factor 8 (FGF-8) is a critical mitogenic factor that is required for normal development of the eye, ear, brain, and limb. FGF8 broadly functions to promote cell proliferation, differentiation, and migration. Overexpression of FGF-8 increases tumor growth and angiogenesis. Human and mouse FGF-8 proteins show 100% homology.
Activity AssayHuman FGF-8 is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of BALB/c 3T3 cells and it is typically less than 150 ng/ml. This corresponds to an expected specific activity of 6.7 x 105 units/mg.
AA SequenceMQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR
Alternative NamesFibroblast Growth Factor 8, FGF8, FGF 8, androgen-induced growth factor, AIGF, heparin-binding growth factor 8, HBGF-8
|Molecular Weight||22.5 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 5 mM sodium phosphate, 50 mM soldium chloride, pH 7.5|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.