Recombinant Human GDF-5 / BMP-14
Induced alkaline phosphates activity in ATDC5 cells (with 2 μg/ml heparin) for Human GDF-5. Alkaline phosphatase activity was measured to calculate the ED50, which is as expected less than 1 μg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human GDF-5 has a predicted Mw of 27.4 kDa (each monomer is 13.7 kDa).
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DescriptionGrowth Differentiation Factor 5 (GDF-5), also known as BMP-14, is a member of the Bone Morphogenic Protein (BMP) and Transforming Growth Factor β (TGF-β) families and functions to regulate cell proliferation and differentiation in embryonic and adult tissues. GDF-5 is expressed in the central nervous system and promotes the survival of dopaminergic neurons in animal models of Parkinson's disease. GDF-5 is also important during chondrogenesis and chondrocyte differentiation.
Activity AssayHuman GDF-5 is fully biologically active when compared to standard. The activity is determined by the ability to induce alkaline phosphatase activity in ATDC5 cells and it is typically between 0.1 - 1 µg/ml. This corresponds to an expected specific activity of 1 x 104 units/mg.
AA SequenceMAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R
Alternative NamesGrowth Differentiation Factor 5, GDF5, GDF 5, Bone Morphogenic Protein 14, BMP14, BMP-14, BMP 14, CDMP-1, CDMP1, SYNS2, cartilage-derived morphogenetic protein 1, cartilage-derived morphogenetic protein-1, radotermin, LAP4, OS5
|Length||121 / 242 aa|
|Molecular Weight||13.7 / 27.4 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.