Recombinant Human GRO-α / CXCL1
Induced chemotaxis of human neutrophils assay for Human GRO-α.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human GRO-α has a predicted Mw of 7.9 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Growth regulated protein α (GRO-α), also known as CXCL1, is a chemokine that has mitogenic properties and is a neutrophil chemoattractant. GRO-α is secreted by macrophages, epithelial cells, neutrophils, and melanomas. GRO-α signals through the CXCR2 chemokine receptor and is important during spinal cord formation, inflammation, angiogenesis, tumorigenesis, and wound healing.Activity Assay
Human GRO-α is fully biologically active when compared to standard. The activity is determined by the induced chemotaxis of human neutrophils.AA Sequence
ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSNAlternative Names
Growth regulated protein α, GROa, GROα| Product Details | |
|---|---|
| Length | 73 aa |
| Molecular Weight | 7.9 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P09341 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
