Recombinant Human Growth Hormone
Induced proliferation of PD11 cells assay for Human Growth Hormone.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Growth Hormone has a predicted Mw of 22.3 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Growth hormone is an important mitogenic growth factor that is synthesized, stored, and secreted by somatotropic cells of the anterior pituitary gland. Growth Hormone stimulates growth, cell reproduction, and cell regeneration. In children, Growth Hormone deficiencies can cause short stature, growth failure, and delayed sexual maturity. Adult Growth Hormone deficiency presents with reduced lean body mass, increased adiposity, reduced muscle strength, and ultimately premature mortality.Activity Assay
Human Growth Hormone is fully biologically active when compared to standard. The activity is determined by a proliferation assay using PD11 cells.AA Sequence
MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GFAlternative Names
Somatotropin, GH, pituitary growth hormone, IGHD1B, GH-N, GHN, hGH-N| Product Details | |
|---|---|
| Length | 192 aa |
| Molecular Weight | 22.3 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P01241 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium bicarbonate, pH 8.0 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
Citations
Nguyen MT, Koo BK, Thi Vu TT, Song JA, Chong SH, Jeong B, Ryu HB, Moh SH, Choe H. Prokaryotic soluble overexpression and purification of bioactive human growth hormone by fusion to thioredoxin, maltose binding protein, and protein disulfide isomerase.
