Recombinant Human HB-EGF (Animal-Free)

Code Description Price Qty
GFH180AF-10 Human HB-EGF (Animal-Free), 10 μg $70.00
GFH180AF-50 Human HB-EGF (Animal-Free), 50 μg $180.00
GFH180AF-100 Human HB-EGF (Animal-Free), 100 μg $320.00
GFH180AF-1000 Human HB-EGF (Animal-Free), 1 mg $1,440.00
Induced proliferation of BALB/c 3T3 cells assay for Human HB-EGF. Cell proliferation was measured to calculate the ED50, which is as expected less than 1 ng/ml.

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Heparin-Binding Epidermal Growth Factor-like growth factor (HB-EGF) is a member of the Epidermal Growth Factor (EGF) family and is expressed by monocytes and macrophages. HB-EGF is the predominant growth factor involved in epithelialization during wound healing. HB-EGF signals through the receptor tyrosine kinase ErbB2 to maintain adult heart homeostasis and promotes cardiac valve development through binding in high affinity to the epidermal growth factor receptor (EGFR). HB-EGF binds the the ErbB4 receptor tyrosine kinase to mediate implantation of the human blastocyst. HB-EGF also functions as a potent mitogen for fibroblasts and smooth muscle cells.

Activity Assay

Human HB-EGF (Animal-Free) is fully biologically active when compared to standard. The activity is determined by a proliferation assay using BALB/c 3T3 cells and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1.0 x 106 units/mg.

AA Sequence

MDLQEADLDL LRVTLSSKPQ ALATPNKEEH GKRKKKGKGL GKKRDPCLRK YKDFCIHGEC KYVKELRAPS CICHPGYHGE RCHGLSL

Alternative Names

Heparin-Binding Epidermal Growth Factor-like growth factor, HBEGF, diphtheria toxin receptor, DT-R
Product Details
Length 87 aa
Molecular Weight 9.9 kDa
Structure Monomer
Source E. coli
Accession Number Q99075
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.