Recombinant Human HB-EGF
Induced proliferation of BALB/c 3T3 cells assay for Human HB-EGF. Cell proliferation was measured to calculate the ED50, which is as expected less than 1 ng/ml.
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DescriptionHeparin-Binding Epidermal Growth Factor-like growth factor (HB-EGF) is a member of the Epidermal Growth Factor (EGF) family and is expressed by monocytes and macrophages. HB-EGF is the predominant growth factor involved in epithelialization during wound healing. HB-EGF signals through the receptor tyrosine kinase ErbB2 to maintain adult heart homeostasis and promotes cardiac valve development through binding in high affinity to the epidermal growth factor receptor (EGFR). HB-EGF binds the the ErbB4 receptor tyrosine kinase to mediate implantation of the human blastocyst. HB-EGF also functions as a potent mitogen for fibroblasts and smooth muscle cells.
Activity AssayHuman HB-EGF is fully biologically active when compared to standard. The activity is determined by a proliferation assay using BALB/c 3T3 cells and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1.0 x 106 units/mg.
AA SequenceMDLQEADLDL LRVTLSSKPQ ALATPNKEEH GKRKKKGKGL GKKRDPCLRK YKDFCIHGEC KYVKELRAPS CICHPGYHGE RCHGLSL
Alternative NamesHeparin-Binding Epidermal Growth Factor-like growth factor, HBEGF, diphtheria toxin receptor, DT-R
|Molecular Weight||9.9 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.