Recombinant Human IFN-α 2b
Viral CPE assay using EMC virus on A549 cells for Human IFN-α 2b.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IFN-α 2b has a predicted Mw of 19.4 kDa.
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DescriptionInterferon-α 2b (IFN-α 2b) is a type I interferon made by leukocytes during viral infection. The JAK-STAT pathway mediates the antiviral and anti-cell proliferation activities of IFN-α 2b. IFN-α proteins are widely used as standard treatments during antiviral and antineoplastic therapies. The IFN-α 2b variant differs from IFN-α 2b by one amino acid.
Activity AssayHuman IFN-α 2b is fully biologically active when compared to standard. The activity is determined by the viral CPE assay using EMC virus on A549 cells. The activity corresponds to an expected specific activity of 2.0 x 108 units/mg.
AA SequenceMCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
Alternative NamesInterferon-α 2b, interferon alpha, interferon-alpha, type I Interferon α 2, IFN α 2
|Molecular Weight||19.4 kDa|
|Accession Number||P01563 (VAR_004012)|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.