Recombinant Human IGF-2 (Animal-Free)
Induced proliferation of mouse FDC-P1 cells assay for Human IGF-2. Cell proliferation was measured to calculate the ED50, which is as expected less than 15 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IGF-2 has a predicted Mw of 7.5 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.Description
Insulin-like Growth Factor 2 (IGF-2) is an important fetal growth hormone made by theca cells during gestation. IGF-2 engages the IGF-1 receptor (IGF1R) to mediate embryonic growth. IGF-2 also binds the sink IGF-2 receptor (IGF2R) leading to IGF-2 degradation.Activity Assay
Human IGF-2 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce mouse FDC-P1 cells proliferation and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.AA Sequence
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSEAlternative Names
Insulin-like Growth Factor 2, somatamedin A, IGF-II| Product Details | |
|---|---|
| Length | 67 aa |
| Molecular Weight | 7.5 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P01344 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
