Recombinant Human IGF-2 (Animal-Free)

Code Description Price Qty
GFH14AF-10 Human IGF-2 (Animal-Free), 10 μg $70.00
GFH14AF-50 Human IGF-2 (Animal-Free), 50 μg $180.00
GFH14AF-100 Human IGF-2 (Animal-Free), 100 μg $320.00
GFH14AF-1000 Human IGF-2 (Animal-Free), 1 mg $1,320.00
Induced proliferation of mouse FDC-P1 cells assay for Human IGF-2. Cell proliferation was measured to calculate the ED50, which is as expected less than 15 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IGF-2 has a predicted Mw of 7.5 kDa.

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Insulin-like Growth Factor 2 (IGF-2) is an important fetal growth hormone made by theca cells during gestation. IGF-2 engages the IGF-1 receptor (IGF1R) to mediate embryonic growth. IGF-2 also binds the sink IGF-2 receptor (IGF2R) leading to IGF-2 degradation.

Activity Assay

Human IGF-2 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce mouse FDC-P1 cells proliferation and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.

AA Sequence

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Alternative Names

Insulin-like Growth Factor 2, somatamedin A, IGF-II
Product Details
Length 67 aa
Molecular Weight 7.5 kDa
Structure Monomer
Source E. coli
Accession Number P01344
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.