Recombinant Human IHH (Animal-Free)

Code Description Price Qty
GFH182AF-5 Human IHH (Animal-Free), 5 μg $70.00
GFH182AF-25 Human IHH (Animal-Free), 25 μg $180.00
GFH182AF-100 Human IHH (Animal-Free), 100 μg $455.00
GFH182AF-1000 Human IHH (Animal-Free), 1 mg $3,005.00
Induced alkaline phosphatase activity of CCL-26 cells assay for Human IHH. Cell proliferation was measured to calculate the ED50, which is in the range of μg/ml.

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Indian hedgehog (IHH) is an essential signaling factor that is secreted in the gut, cartilage, and bone during embryonic development. IHH acts through the patched (PTC) receptor to induce transcriptional changes important for bone and cartilage development. IHH also induces the expression of parathyroid hormone-related peptide (PTHrP), which in turn mediates IHH activity during chondrocyte differentiation, forming a negative feedback loop.

Activity Assay

Human IHH (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the induced alkaline phosphatase activity of CCL-26 cells.

AA Sequence

MIIGPGRVVG SRRRPPRKLV PLAYKQFSPN VPEKTLGASG RYEGKIARSS ERFKELTPNY NPDIIFKDEE NTGADRLMTQ RCKDRLNSLA ISVMNQWPGV KLRVTEGWDE DGHHSEESLH YEGRAVDITT SDRDRNKYGL LARLAVEAGF DWVYYESKAH VHCSVKSEHS AAAKTGG

Alternative Names

Indian Hedgehog, HHG-2
Product Details
Length 177 aa
Molecular Weight 19.9 kDa
Structure Monomer
Source E. coli
Accession Number Q14623
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.