Recombinant Human IHH (Animal-Free)
Induced alkaline phosphatase activity of CCL-26 cells assay for Human IHH. Cell proliferation was measured to calculate the ED50, which is in the range of μg/ml.
Achieve Purchasing Synergy
Request a quote at [email protected] for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order.
PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.Description
Indian hedgehog (IHH) is an essential signaling factor that is secreted in the gut, cartilage, and bone during embryonic development. IHH acts through the patched (PTC) receptor to induce transcriptional changes important for bone and cartilage development. IHH also induces the expression of parathyroid hormone-related peptide (PTHrP), which in turn mediates IHH activity during chondrocyte differentiation, forming a negative feedback loop.Activity Assay
Human IHH (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the induced alkaline phosphatase activity of CCL-26 cells.AA Sequence
MIIGPGRVVG SRRRPPRKLV PLAYKQFSPN VPEKTLGASG RYEGKIARSS ERFKELTPNY NPDIIFKDEE NTGADRLMTQ RCKDRLNSLA ISVMNQWPGV KLRVTEGWDE DGHHSEESLH YEGRAVDITT SDRDRNKYGL LARLAVEAGF DWVYYESKAH VHCSVKSEHS AAAKTGGAlternative Names
Indian Hedgehog, HHG-2| Product Details | |
|---|---|
| Length | 177 aa |
| Molecular Weight | 19.9 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | Q14623 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
