Recombinant Human IL-1 α (Animal-Free)

Code Description Price Qty
GFH173AF-2 Human IL-1 α (Animal-Free), 2 μg $70.00
GFH173AF-10 Human IL-1 α (Animal-Free), 10 μg $180.00
GFH173AF-100 Human IL-1 α (Animal-Free), 100 μg $700.00
GFH173AF-1000 Human IL-1 α (Animal-Free), 1 mg $4,205.00
Induced proliferation of D10.G4.1 cells assay for Human IL-1 α. Cell proliferation was measured to calculate the ED50, which is as expected in the range of pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-1 α has a predicted Mw of 18 kDa.

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Interleukin-1 α (IL-1 α) is expressed by epithelial cells, activated macrophages, neutrophils, and endothelial cells to regulate immune responses. IL-1 α signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1 α and the independently regulated IL-1 β protein have overlapping proinflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.

Activity Assay

Human IL-1 α is fully biologically active when compared to standard. The activity is determined by the ability to induce D10.G4.1 cells proliferation.

AA Sequence

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Alternative Names

Interleukin-1 α, IL-1F1, IL1, FAF, BAF, LEM, LAF
Product Details
Length 159 aa
Molecular Weight 18 kDa
Structure Monomer
Source E. coli
Accession Number P01583
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.