Recombinant Human IL-1 β (Animal-Free)
Induced proliferation of D10S cells assay for Human IL-1 β. Cell proliferation was measured to calculate the ED50, which is as expected less than 12 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-1 β has a predicted Mw of 17.5 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.Description
Interleukin-1 β (IL-1 β) is a pro-inflammatory cytokine that is produced by monocytes, macrophages, and dendritic cells (DCs). IL-1 β signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1 β and the independently regulated IL-1 α protein have overlapping proinflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.Activity Assay
Human IL-1 β (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce D10S cells proliferation and it is typically less than 12 pg/ml. This corresponds to an expected specific activity of 8.3 x 107 units/mg.AA Sequence
MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG GQDITDFTMQ FVSSAlternative Names
Interleukin-1 β, preinterleukin 1 β, IL1, IL 1, catabolin, LAF, EP, LEM, MCF| Product Details | |
|---|---|
| Length | 154 aa |
| Molecular Weight | 17.5 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P01584 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
Nakashima KI, Ogiwara T, Hirai T, Tanaka T, Murata H, Kaburagi K, Fujii-Kuriyama Y, Hayashi H, Inoue M. Gerontoxanthone B from Maclura cochinchinensis var. gerontogea exhibits anti-inflammatory potential as an aryl hydrocarbon receptor agonist. (2017).
