Recombinant Human IL-10
Induced proliferation of MC/9 cells for Human IL-10. Cell proliferation was measured to calculate the ED50, which is as expected less than 5 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-10 has a predicted Mw of 18.8 kDa.
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DescriptionInterleukin-10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon-γ (IFN-γ), Tumor Necrosis Factor α (TNF-α), interleukin-2 (IL-2), interleukin-3 (IL-3), interleukin-4 (IL-4), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Human IL-10 can act on mouse cells, while mouse IL-10 is not active on human cells.
Activity AssayHuman IL-10 is fully biologically active when compared to standard. The activity is determined by the ability to induce MC/9 cells proliferation and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2 x 105 units/mg.
AA SequenceMSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGEKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLQDQGVYKA MNEFDIFINC IEAYMMIKMK S
Alternative NamesInterleukin-10, interleukin 10, IL10, IL 10
|Molecular Weight||18.8 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.