Recombinant Human IL-17A
Induced production of Mouse IL-6 by 3T3 cells. Production of mouse IL-6 was measured to calculate the ED50, which is as expected less than 10 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-17A has a predicted Mw of 31.3 kDa (each monomer is 15.1 kDa).
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Interleukin-17A (IL-17A), also known as CTLA-8, is a member of the IL-17 family of proteins. IL-17A is a proinflammatory cytokine that is secreted by activated CD4+ and CD8+ T lymphocytes. IL-17A acts through its receptor, IL-17R, to promote increased cytokine and chemokine secretion. In turn, the cytokines and chemokines mediate the immunoregulatory function of IL-17A by promoting the proliferation, maturation, and chemoattraction of neutrophils to inflammatory sites. Elevated levels of IL-17A are associated with rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer, and multiple sclerosis. Human, mouse, and rat IL-17A show activity on mouse cells.Activity Assay
Human IL-17A is fully biologically active when compared to standard. The activity is determined by the ability to produce mouse IL-6 by 3T3 cells and it is typically less than 10 ng/ml. This corresponds to an expected specific activity of 1 x 105 units/mg.AA Sequence
MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVAAlternative Names
Interleukin-17A, interleukin 17A, IL17A, IL 17A| Product Details | |
|---|---|
| Length | 137 / 274 aa |
| Molecular Weight | 15.7 / 31.3 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | Q16552 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
