Recombinant Human IL-27 / EBI-3 (Animal-Free)

Code Description Price Qty
GFH162AF-5 Human IL-27 / EBI-3 (Animal-Free), 5 μg $70.00
GFH162AF-20 Human IL-27 / EBI-3 (Animal-Free), 20 μg $180.00
GFH162AF-100 Human IL-27 / EBI-3 (Animal-Free), 100 μg $695.00
GFH162AF-1000 Human IL-27 / EBI-3 (Animal-Free), 1 mg $5,765.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-27 / EBI-3 has a predicted Mw of 23.4 kDa.

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Epstein-Barr virus induced gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family related to the p40 subunit of interleukin-12 (IL-12). EBI-3 expression is induced in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form interleukin-27 (IL-27), and with p35 to form interleukin-35 (IL-35). Both IL-27 and IL-35 have anti-inflammatory and regulatory activity.

Activity Assay

Activity to be determined.

AA Sequence

MRKGPPAALT LPRVQCRASR YPIAVDCSWT LPPAPNSTSP VSFIATYRLG MAARGHSWPC LQQTPTSTSC TITDVQLFSM APYVLNVTAV HPWGSSSSFV PFITEHIIKP DPPEGVRLSP LAERQLQVQW EPPGSWPFPE IFSLKYWIRY KRQGAARFHR VGPIEATSFI LRAVRPRARY YVQVAAQDLT DYGELSDWSL PATATMSLGK

Alternative Names

IL-35/EBI-3, IL-27/EBI-3, interleukin-27, interleukin 27, IL27, IL 27, EBI-3 subunit, epstein-barr virus induced gene-3
Product Details
Length 210 aa
Molecular Weight 23.4 kDa
Structure Monomer
Source E. coli
Accession Number Q14213
Purity ≥90% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤5.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) and mannitol (2:1 mannitol to protein ratio)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 20 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.