Recombinant Human IL-3 (Animal-Free)

Code Description Price Qty
GFH80AF-2 Human IL-3 (Animal-Free), 2 μg $70.00
GFH80AF-10 Human IL-3 (Animal-Free), 10 μg $180.00
GFH80AF-100 Human IL-3 (Animal-Free), 100 μg $455.00
GFH80AF-1000 Human IL-3 (Animal-Free), 1 mg $3,245.00
Induced proliferation of TF-1 cells assay for Human IL-3. Cell proliferation was measured to calculate the ED50, which is as expected less than 2 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-2 has a predicted Mw of 15.2 kDa.

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Interleukin-3 (IL-3) is a cytokine that is produced by activated T cells and mast cells. IL-3 induces the differentiation of hematopoietic stem cells into myeloid precursor cells, such as erythrocyte, megakaryocyte, granulocyte, monocyte, and dendritic cells. IL-3 also functions in the nervous system and is important during the B-1 cell regulation of chronic inflammatory diseases.

Activity Assay

Human IL-3 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5.0 x 105 units/mg.

AA Sequence

MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

Alternative Names

Interleukin-3, interleukin 3, IL3, IL 3, MCGF, Multi-CSF, HCGF, P-cell stimulation factor
Product Details
Length 134 aa
Molecular Weight 15.2 kDa
Structure Monomer
Source E. coli
Accession Number P08700
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.