Recombinant Human IL-32 α

Code Description Price Qty
GFH151-2 Human IL-32 α, 2 μg $65.00
GFH151-10 Human IL-32 α, 10 μg $165.00
GFH151-100 Human IL-32 α, 100 μg $635.00
GFH151-1000 Human IL-32 α, 1 mg $5,240.00
Induced production of TNF-α from human PBMC cells for Human IL-32 α. Cell proliferation was measured to calculate the ED50.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-32 α has a predicted Mw of 14.9 kDa.

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Description

Interleukin-32 α (IL-32 α) is one of six known splice variants of the IL-32 gene. IL-32 α induces the macrophage production of inflammatory cytokines, such as interleukin-8 (IL-8), Tumor Necrosis Factor α (TNF-α), and Macrophage Inflammatory Protein 2 (MIP-2). IL-32 α expression is increased after the activation of T cells, Natural Killer (NK) cells, and interferon γ-treated epithelial cells.

Activity Assay

Human IL-32 α is fully biologically active when compared to standard. The activity is determined by the induced production of TNF-α from human PBMC cells.

AA Sequence

MTCSLSFGAP ISKEDLRTTI DLLKQESQDL YNNYSIKQAS GMSADESIQL PCFSLDREAL TNISVIIAHL EKVKVLSENT VDTSWVIRWL TNISCFNPLN LNISVPGNTD ESYDCKVFVL TVLKQFSNCM AELQAKDNTT C

Alternative Names

Interleukin-31, interleukin 31, IL31, IL 31
Product Details
Length 141 aa
Molecular Weight 15.7 kDa
Structure Monomer
Source E. coli
Accession Number Q6EAL8
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.