Recombinant Human IL-32 α
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DescriptionInterleukin-32 α (IL-32 α) is one of six known splice variants of the IL-32 gene. IL-32 α induces the macrophage production of inflammatory cytokines, such as interleukin-8 (IL-8), Tumor Necrosis Factor α (TNF-α), and Macrophage Inflammatory Protein 2 (MIP-2). IL-32 α expression is increased after the activation of T cells, Natural Killer (NK) cells, and interferon γ-treated epithelial cells.
Activity AssayHuman IL-32 α is fully biologically active when compared to standard. The activity is determined by the induced production of TNF-α from human PBMC cells.
AA SequenceMTCSLSFGAP ISKEDLRTTI DLLKQESQDL YNNYSIKQAS GMSADESIQL PCFSLDREAL TNISVIIAHL EKVKVLSENT VDTSWVIRWL TNISCFNPLN LNISVPGNTD ESYDCKVFVL TVLKQFSNCM AELQAKDNTT C
Alternative NamesInterleukin-31, interleukin 31, IL31, IL 31
|Molecular Weight||15.7 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.