Recombinant Human IL-35

Code Description Price Qty
GFH49-2 Human IL-35, 2 μg $65.00
GFH49-10 Human IL-35, 10 μg $245.00
GFH49-100 Human IL-35, 100 μg $1,965.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Single chai, HIS-tagged Human IL-35 has a predicted Mw of 49 kDa, but shows approximately 75 - 95 kDa under re

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Description

Interleukin-35 (IL-35) is a member of the IL-12 cytokine family and is produced by regulatory T cells (Tregs). IL-35 is a heterodimeric cytokine that is comprised of the interleukin-12 subunit P35 (IL-12 α) and the interleukin-27 subunit β (IL-27 β) chains. IL-35 binds the IL-12Rβ2/gp130 hetero- and homodimers to activate STAT1 and STAT4 signaling. IL-35 functions as a suppressor of immune cell inflammatory responses.

Activity Assay

There is no biological assay data available but protein content is validated by western blot.

AA Sequence

EBI-3: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK p35: RNLPVATPDP G

Alternative Names

Interleukin-35, interleukin 35, IL35, IL 35
Product Details
Length 406 aa
Molecular Weight 45.8 / 60-65 unreduced, 70-80 reduced kDa
Structure Single chain dimer
Source HEK 293 cells
Accession Number Q14213/P29459
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤5.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 30 mM sodium chloride, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.