Recombinant Human IL-4

Code Description Price Qty
GFH9-5 Human IL-4, 5 μg $65.00
GFH9-20 Human IL-4, 20 μg $165.00
GFH9-100 Human IL-4, 100 μg $425.00
GFH9-1000 Human IL-4, 1 mg $3,495.00
Induced proliferation of TF-1 cells assay for Human IL-4.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-4 has a predicted Mw of 15.1 kDa.

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PODS® Growth Factors

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Description

Interleukin-4 (IL-4) is an immunomodulatory cytokine that functions to induce naive helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.

Activity Assay

Human IL-4 is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 250 pg/ml. This corresponds to an expected specific activity of 4 x 106 units/mg.

AA Sequence

MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Alternative Names

Interleukin-4, interleukin 4, IL4, IL 4, B cell stimulating factor, BSF-1, BCDF, BCGF, pitrakinra, BSF11
Product Details
Length 130 aa
Molecular Weight 15.1 kDa
Structure Monomer
Source E. coli
Accession Number P05112
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.