Recombinant Human IL-4
Induced proliferation of TF-1 cells assay for Human IL-4.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-4 has a predicted Mw of 15.1 kDa.
Achieve Purchasing Synergy
Request a quote at [email protected] for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order.
PODS® Growth Factors
This growth factor is also available as PODS® for sustained release, see here.Description
Interleukin-4 (IL-4) is an immunomodulatory cytokine that functions to induce naive helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.Activity Assay
Human IL-4 is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 250 pg/ml. This corresponds to an expected specific activity of 4 x 106 units/mg.AA Sequence
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSSAlternative Names
Interleukin-4, interleukin 4, IL4, IL 4, B cell stimulating factor, BSF-1, BCDF, BCGF, pitrakinra, BSF11| Product Details | |
|---|---|
| Length | 130 aa |
| Molecular Weight | 15.1 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P05112 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
