Recombinant Human IL-5 (Animal-Free)

Code Description Price Qty
GFH191AF-2 Human IL-5 (Animal-Free), 2 μg $70.00
GFH191AF-10 Human IL-5 (Animal-Free), 10 μg $180.00
GFH191AF-100 Human IL-5 (Animal-Free), 100 μg $585.00
GFH191AF-1000 Human IL-5 (Animal-Free), 1 mg $4,805.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-5 has a predicted Mw of 26.6 kDa (each monomer is 13.3 kDa).

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Interleukin-5 (IL-5) is a hematopoietic growth factor that is expressed in type 2 T helper (Th2) cells, mast cells, and eosinophils. IL-5 acts through the IL-5 receptor (IL-5R), stimulates B cell growth, and mediates eosinophil activation. Human and mouse IL-5 show cross-reactivity.

Activity Assay

Human IL-5 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 250 pg/ml. This corresponds to an expected specific activity of 4 x 106 units/mg.

AA Sequence

MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

Alternative Names

Interleukin-5, interleukin 5, IL5, IL 5, B-cell differentiation factor I, eosinophil differentiation factor, TFR
Product Details
Length 116 / 232 aa
Molecular Weight 13.3 / 26.6 kDa
Structure Dimer
Source E. coli
Accession Number P05113
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium glycinate, pH 8.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.