Recombinant Human IL-9

Code Description Price Qty
GFH82-2 Human IL-9, 2 μg £50.00
GFH82-10 Human IL-9, 10 μg £120.00
GFH82-100 Human IL-9, 100 μg £445.00
GFH82-1000 Human IL-9, 1 mg £3,690.00
Induced proliferation of MO7e cells for Human IL-9. Cell proliferation was measured to calculate the ED50, which is as expected less than 5 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-9 has a predicted Mw of 14.3 kDa.

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Description

Interleukin-9 (IL-9) is a cytokine produced by type 2 T helper (Th2) cells and regulates hematopoietic cells. IL-9 signals through the interleukin-9 receptor (IL9R) to activate STAT signaling. IL-9 functions to induce cell proliferation, prevent cell apoptosis, and is associated with asthma and airway hyperresponsiveness.

Activity Assay

Human IL-9 is fully biologically active when compared to standard. The activity is determined by the ability to induce MO7e cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5 x 105 units/mg.

AA Sequence

MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI

Alternative Names

Interleukin-9, interleukin 9, IL9, IL 9
Product Details
Length 127 aa
Molecular Weight 14.3 kDa
Structure Monomer
Source E. coli
Accession Number P15248
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.