Recombinant Human IP-10 / CXCL10
Induced chemotaxis of primary human T cells assay for Human IP-10. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Human IP-10 starting at 5 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IP-10 has a predicted Mw of 8.6 kDa.
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DescriptionInterferon γ-induced protein 10 (IP-10), also known as CXCL10, is a chemokine secreted by monocytes, endothelial cells and fibroblasts in response to interferon γ (IFN-γ). IP-10 functions as a chemoattractant for activated T cells, monocytes, dendritic, and Natural Killer (NK) cells that express the G protein-coupled receptor CXCR3. IP-10 is an important factor in autoimmune diseases such as Hashimoto's thyroiditis, Graves' disease, and Type 1 diabetes mellitus.
Activity AssayHuman IP-10 is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of human primary T cells.
AA SequenceVPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP
Alternative NamesMonocyte Chemotactic Protein 1, CCL2, JE, MCAF
|Molecular Weight||8.6 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.