Recombinant Human LIF

Code Description Price Qty
GFH200-5 Human LIF, 5 μg $50.00
GFH200-20 Human LIF, 20 μg $140.00
GFH200-100 Human LIF, 100 μg $300.00
GFH200-1000 Human LIF, 1 mg $1,640.00

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PODS® Growth Factors

This growth factor is also available as PODS® for sustained release, see here.

Description

Leukemia Inhibitory Factor (LIF) is a member of the interleukin-6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. Human LIF may also be an important factor during human embryonic stem cell (hESC) self-renewal, pluripotency, and embryonic implantation.

Activity Assay

Specific activity of the sample is approximate 108 units/mg. 106 units, identical 10 μg of pure protein, are sufficient to treat 1.0 L of stem cells including human embryonic stem cells, hematopoietic stem cells, mesenchymal stem cells, neural stem cells and induced pluripotent stem cells.

AA Sequence

MSPLPITPVN ATCAIRHPCH NNLMNQIRSQ LAQLNGSANA LFILYYTAQG EPFPNNLDKL CGPNVTDFPP FHANGTEKAK LVELYRIVVY LGTSLGNITR DQKILNPSAL SLHSKLNATA DILRGLLSNV LCRLCSKYHV GHVDVTYGPD TSGKDVFQKK KLGCQLLGKY KQIIAVLAQA F

Alternative Names

Leukocyte Inhibitory Factor, leukemia inhibitory factor, cholinergic differentiation factor
Product Details
Length 181 aa
Molecular Weight 19.8 kDa
Structure Monomer
Source E. coli
Accession Number P09056
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a volatile solution after reverse phase chromatography.
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.