Recombinant Human MCP-4 / CCL13
Induced chemotaxis of THP-1 cells assay for Human MCP-4. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Human MCP-4 starting at 1000 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human MCP-4 has a predicted Mw of 8.6 kDa.
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DescriptionMonocyte Chemotactic Protein 4 (MCP-4), also known as CCL13, is induced by inflammatory proteins such as interleukin-1 (IL-1) and Tumor Necrosis Factor α (TNF-α). MCP-4 is a ligand for the G protein coupled chemokine receptors CCR2, CCR3, and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils, and basophils during inflammation and allergic responses.
Activity AssayHuman MCP-4 is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of THP-1 cells.
AA SequenceQPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT
Alternative NamesMonocyte Chemotactic Protein 1, CCL2, JE, MCAF
|Molecular Weight||8.6 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.