Recombinant Human MIP-3 β / CCL19
Induced chemotaxis of primay human T cells assay for Human MIP-3 β. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Human MIP-3 β starting at 5 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human MIP-3 β has a predicted Mw of 8.8 kDa. Two different batches are represented.
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DescriptionMacrophage Inflammatory Protein 3 β (MIP-3 β), also known as CCL19, is a chemokine that is expressed in the thymus, lymph nodes, and activated bone marrow stromal cells. MIP-3 β signals through the G protein-coupled receptor CCR7 to regulate normal lymphocyte recirculation. MIP-3 β also functions during T cell trafficking to the thymus, and in T cell and B cell homing to the lymph nodes and secondary lymphoid organs. Human MIP-3 β shows activity on mouse cells.
Activity AssayHuman MIP-3 β is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of primary human T cells.
AA SequenceGTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS
Alternative NamesMonocyte Chemotactic Protein 1, CCL2, JE, MCAF
|Molecular Weight||8.8 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.