Recombinant Human NT-3

Code Description Price Qty
GFH99-2 Human NT-3, 2 μg £55.00
GFH99-10 Human NT-3, 10 μg £130.00
GFH99-100 Human NT-3, 100 μg £470.00
GFH99-1000 Human NT-3, 1 mg £3,875.00
Induced proliferation of C6 cells assay for Human NT-3.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human NT-3 has a predicted Mw of 27.2 kDa (each monomer is 13.6 kDa).

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PODS® Growth Factors

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Description

Neurotrophin-3 (NT-3) is an important member of the Nerve Growth Factor (NGF) family of proteins. NT-3 promotes the growth, survival, and differentiation of neurons and synapses in the peripheral and central nervous systems. The receptor tyrosine kinase TrkC exclusively binds in high-affinity to NT-3. NT-3 also signals through the receptor tyrosine kinase TrkB, and through the low affinity nerve growth factor receptor (LNGFR).

Activity Assay

Human NT-3 is fully biologically active when compared to standard. The activity is determined by the ability to induce C6 cells proliferation.

AA Sequence

MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT

Alternative Names

Migration Inhibitory Factor, GIF, phenylpyruvate tautomerase, glycosylation-inhibiting factor, L-dopachrome tautomerase
Product Details
Length 120 / 240 aa
Molecular Weight 13.8 / 27.5 kDa
Structure Dimer
Source E. coli
Accession Number P20783
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.