Recombinant Human PDGF-AB

Code Description Price Qty
GFH17-2 Human PDGF-AB, 2 μg $65.00
GFH17-10 Human PDGF-AB, 10 μg $165.00
GFH17-100 Human PDGF-AB, 100 μg $465.00
GFH17-1000 Human PDGF-AB, 1 mg $3,820.00
Induced proliferation of NR6R-3T3 cells assay for Human PDGF-AB. Cell proliferation was measured to calculate the ED50, which is as expected less than 20 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human PDGF-AB has a predicted Mw of 26.8 kDa (α chain is 14.4 kDa and β chain is 12.4 kDa).

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Description

Platelet-Derived Growth Factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the α-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b.

Activity Assay

Human PDGF-AB is fully biologically active when compared to standard. The activity is determined by the ability to induce NR6R-3T3 cells proliferation and it is typically less than 20 ng/ml. This corresponds to an expected specific activity of 5 x 104 units/mg.

AA Sequence

Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT

Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVR

Alternative Names

Platelet-Derived Growth Factor, GDGF, ODGF, PDGF AB
Product Details
Length Alpha: 126, Beta: 110, Total: 236 aa
Molecular Weight Alpha: 14.4, Beta: 12.4, Total: 26.8 kDa
Structure Dimer
Source E. coli
Accession Number P04085/P01127
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.