Recombinant Human RANK Ligand

Code Description Price Qty
GFH19-2 Human RANK Ligand, 2 μg $65.00
GFH19-10 Human RANK Ligand, 10 μg $165.00
GFH19-100 Human RANK Ligand, 100 μg $635.00
GFH19-1000 Human RANK Ligand, 1 mg $5,240.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human RANK Ligand has a predicted Mw of 19.7 kDa.

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PODS® Growth Factors

This growth factor is also available as PODS® for sustained release, see here.

Description

Receptor activator of nuclear factor kappa-B Ligand (RANK Ligand) is a cell-bound marker related to the Tumor Necrosis Factor (TNF) family of proteins. RANK Ligand plays a critical role in bone metabolism and osteoclast differentiation. T cell expression of RANK Ligand promotes dendritic cell maturation.

Activity Assay

Human RANK Ligand (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the activity of RAW-Blue™ cells and it is typically less than 50 ng/ml. This corresponds to an expected specific activity of 2.0 x 104 units/mg.

AA Sequence

EKAMVDGSWL DLAKRSKLEA QPFAHLTINA TDIPSGSHKV SLSSWYHDRG WAKISNMTFS NGKLIVNQDG FYYLYANICF RHHETSGDLA TEYLQLMVYV TKTSIKIPSS HTLMKGGSTK YWSGNSEFHF YSINVGGFFK LRSGEEISIE VSNPSLLDPD QDATYFGAFK VRDID

Alternative Names

Receptor activator of nuclear factor kappa-B Ligand, RANKL, RANK-L, RANK-ligand, TNFSF11, TRANCE, OPGL, ODF
Product Details
Length 175 aa
Molecular Weight 19.7 kDa
Structure Monomer
Source E. coli
Accession Number O14788
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.