Recombinant Human RANTES / CCL5
Induced chemotaxis of human neutrophils assay for Human RANTES. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Human RANTES starting at 1000 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human RANTES has a predicted Mw of 7.9 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Regulated upon activation, normal T cell expressed and secreted (RANTES), also known as CCL5, is a chemokine produced by T cells three to five days after T cell activation. RANTES signals through G protein-coupled receptors CCR5, CCR3, CCR1, and through the human CMV-encoded viral receptor US28. RANTES functions to recruit immune cells to inflammatory sites.Activity Assay
Human RANTES is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of human neutrophils.AA Sequence
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMSAlternative Names
CCL5, TCP228, SIS-delta, SCYA5| Product Details | |
|---|---|
| Length | 68 aa |
| Molecular Weight | 7.9 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P13501 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
