Recombinant Human SCF (Animal-Free)
Induced proliferation of TF-1 cells assay for Human SCF. Cell proliferation was measured to calculate the ED50, which is as expected less than 15 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human SCF has a predicted Mw of 18.6 kDa.
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PODS® Growth Factors
This growth factor is also available as PODS® for sustained release, see here.Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.Description
Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells.Activity Assay
Human SCF (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of TF-1 cells and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.AA Sequence
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVAAlternative Names
Stem Cell Factor, c-Kit Ligand, KL, steel factor, MGF| Product Details | |
|---|---|
| Length | 165 aa |
| Molecular Weight | 18.6 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P21583 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM soldium chloride, pH 7.5 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
