Recombinant Human SCF
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PODS® Growth FactorsThis growth factor is also available as PODS® for sustained release, see here.
DescriptionStem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells.
Activity AssayHuman SCF is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of TF-1 cells and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.
AA SequenceMEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Alternative NamesStem Cell Factor, c-Kit Ligand, KL, steel factor, MGF
|≥95% determined by reducing and non-reducing SDS-PAGE
|≤1.00 EU/μg as measured by kinetic LAL
|Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM soldium chloride, pH 7.5
|Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
|Stability and Storage
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.