Recombinant Human TGF-α
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PODS® Growth Factors
This growth factor is also available as PODS® for sustained release, see here.Description
Tumor growth factor alpha (TGF-α) is a member of the epidermal growth factor (EGF) family. TGF-α function is mediated through binding the EGF receptor (EGFR) to activate receptor tyrosine kinase signaling. TGF-α functions as a mitogen to activate epithelial cell proliferation, growth, and differentiation. In the gastric mucosa, TGF-α production inhibits gastric acid secretion and therefore plays a central role in the pathogenesis of the stomach disease. TGF-α is also produced in adult macrophages, brain cells, keratinocytes, and is widely expressed in cancer cells.Activity Assay
Human TGF-α is fully biologically active when compared to standard. The activity is determined by the ability to induce 3T3 cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity higher than 5 x 105 units/mg.AA Sequence
VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLAAlternative Names
Tumor growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGFA, TGF type 1| Product Details | |
|---|---|
| Length | 50 aa |
| Molecular Weight | 5.6 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P01135 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
