Recombinant Human TGF-α
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PODS® Growth FactorsThis growth factor is also available as PODS® for sustained release, see here.
DescriptionTumor growth factor alpha (TGF-α) is a member of the epidermal growth factor (EGF) family. TGF-α function is mediated through binding the EGF receptor (EGFR) to activate receptor tyrosine kinase signaling. TGF-α functions as a mitogen to activate epithelial cell proliferation, growth, and differentiation. In the gastric mucosa, TGF-α production inhibits gastric acid secretion and therefore plays a central role in the pathogenesis of the stomach disease. TGF-α is also produced in adult macrophages, brain cells, keratinocytes, and is widely expressed in cancer cells.
Activity AssayHuman TGF-α is fully biologically active when compared to standard. The activity is determined by the ability to induce 3T3 cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity higher than 5 x 105 units/mg.
AA SequenceVVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
Alternative NamesTumor growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGFA, TGF type 1
|Molecular Weight||5.6 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.