Recombinant Human TNF-α (Animal-Free)

Code Description Price Qty
GFH111AF-10 Human TNF-α (Animal-Free), 10 μg $70.00
GFH111AF-50 Human TNF-α (Animal-Free), 50 μg $180.00
GFH111AF-100 Human TNF-α (Animal-Free), 100 μg $320.00
GFH111AF-1000 Human TNF-α (Animal-Free), 1 mg $1,440.00
Cytolysis of mouse L929 cells in the presence of Actinomycin D for Human TNF-α. Cell viability was measured to calculate the ED50, which is as expected less than 2 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TNF-α has a predicted Mw of 17.5 kDa.

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Tumor Necrosis Factor α (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and Natural Killer (NK) cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

Activity Assay

Human TNF-α (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D and it is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5.0 x 105 units/mg.

AA Sequence

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Alternative Names

Tumor Necrosis Factor α, TNFa, TNFα, TNFSF2, cachectin, DIF, necrosin, cytotoxin, cachexin, TNF
Product Details
Length 158 aa
Molecular Weight 17.5 kDa
Structure Monomer
Source E. coli
Accession Number P01375
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed.

Avoid freeze-thaw cycles