Recombinant Human TNF Receptor Type 1

Code Description Price Qty
GFH35-5 Human TNF Receptor Type 1, 5 μg $65.00
GFH35-20 Human TNF Receptor Type 1, 20 μg $165.00
GFH35-100 Human TNF Receptor Type 1, 100 μg $410.00
GFH35-1000 Human TNF Receptor Type 1, 1 mg $2,950.00
Neutralization of human TNF-α induced proliferation of L929 cells in the presence of Actinomycin D. Cell proliferation was measured to calculate the ED50, which is as expected less than 100 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TNF Receptor Type 1 has a predicted Mw of 18.3 kDa.

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Description

Tumor Necrosis Factor Receptor type 1 (TNF Receptor 1) is expressed in most tissues and is activated by soluble and membrane-bound Tumor Necrosis Factor α (TNF-α). TNF Receptor 1 activates NF-kappaB and MAPK pathways to induce inflammation, promote apoptotic cell death, inhibit tumorigenesis, and inhibit viral replication.

Activity Assay

Human TNF Receptor Type 1 is fully biologically active when compared to standard. The activity is determined by the neutralization of human TNF-α and it is typically less than 100 ng/ml. This corresponds to an expected specific activity of 1.0 x 104 units/mg.

AA Sequence

MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCKN QYRHYWSENL FQCFNCSLCL NGTVHLSCQE KQNTVCTCHA GFFLRENECV SCSNCKKSLE CTKLCLPQIE N

Alternative Names

TNFR1, TNF-receptor-1
Product Details
Length 162 aa
Molecular Weight 18.3 kDa
Structure Monomer
Source E. coli
Accession Number P19438
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.